URINARY 17-KETOSTEROID CONJUGATES IN HIRSUTISM

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URINARY 17 - KETOSTEROID CONJUGATES IN HIRSUTISM By PIERRE MAUVAIS - JARVIS AND ETIENNE - EMILE BAULIEU

The principal urinary 17-ketosteroids (17KS) were analyzed in 16 cases of hirsutism; their conjugated forms were separately cleaved, and the liberated 17KS were chromatographed and quantitated. Six were clinically classified as cases of Stein-Leventhal syndrome and the remaining 10 as "idiopathic hirsutism." 1 The following conditions were excluded a priori: adrenal and ovarian tumors, Cushing'...

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Isolation of urinary 17-ketosteroid conjugates by ammonium sulfate precipitation.

Ammonium sulfate precipitation of urinary 17-ketosteroid conjugates is a rapid, effective method for isolating and purifying them. Negligible quantities of pigment are produced during acid hydrolysis of solutions of the precipitated conjugates. Despite elimination of nonspecific chromogenic material, colorimetric determinations of Zimmerman chromogens with use of the Allen correction of absorba...

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Characteristics of a 17-ketosteroid reaction.

An improved method for the determination of 17-ketosteroids is described, which involves an increase in sensitivity by the simple expedient of upsetting the equilibrium of the reaction in removing the products of reaction by precipitating the chromophore formed. This precipitation system appears to achieve about 1.6 times the color sensitivity for the same reaction carried out as a solution pro...

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Effect of Cortisone on 17-Ketosteroid Excretion *

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ژورنال

عنوان ژورنال: Journal of Clinical Investigation

سال: 1962

ISSN: 0021-9738

DOI: 10.1172/jci104626